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  • DnaD Protein of Bacillus subtilis Interacts with DnaA, the

    The Bacillus subtilis proteins DnaD and DnaB are essential for replication initiation and are conserved in low G+C content Gram-positive bacteria. Ordered association of helicase loader

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  • Co-directional replication-transcription conflicts lead to

    Aug 01, 2015 · Association of helicase loader proteins DnaD and DnaB with rrn loci depends on transcription and the replication restart protein priA. Wild type cells (AG174) and the priA-ssrA * mutant (WKS338) were grown to mid-exponential phase in LB medium.

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  • The Bacillus subtilis DnaD protein: A putative link

    The Bacillus subtilis DnaD protein is an essential protein and a component of the oriC and PriA primosomal cascades, which are responsible for loading the …

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  • Mechanisms for initiating cellular DNA replication | Science

    Feb 24, 2017 · Although the use of a dedicated DnaC/DnaI-family helicase loader represents the textbook view of replication initiation in bacteria, there are a number of bacterial species (perhaps a majority) that appear to lack such proteins (23, 24, 102).

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  • functional interaction between the putative primosomal

    Feb 15, 2002 · If DnaI plays the role of the helicase loader in Bacillus then it should be able to interact with the main replicative hexameric helicase. Although an interaction between DnaI and the replicative helicase was detected previously in B.subtilis by yeast two-hybrid technology ( 18 ), no formation of a stable complex between the two proteins has

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  • Ordered association of helicase loader proteins with the

    Jan 01, 2010 · One important consequence of the ordered association of helicase loading proteins described here is to ensure spatial regulation of replication initiation. Replication initiation is thought to take place at the inner face of the membrane. In E. coli, DnaA interacts with the membrane directly (Garner et al., 1998).

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  • Helicase Flashcards | Quizlet

    The tau proteins are apart of the clamp loader which connects to the leading and lagging strand polymerases. Helicases are called DNA B in Bacteria while just Helicases in Eukaryotes. DNA C is a Protein required to load Helicase around the DNA lagging strand.

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  • Structural Analysis of Bacillus subtilis SPP1 Phage

    Apr 25, 2003 · The Bacillus subtilis SPP1 phage-encoded protein G39P is a loader and inhibitor of the phage G40P replicative helicase involved in the initiation of DNA replication.We have carried out a full x-ray crystallographic and preliminary NMR analysis of G39P and functional studies of the protein, including assays for helicase binding by a number of truncated mutant forms, in an effort to improve …

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  • DnaB, DnaD and DnaI proteins are components of the

    Restart in B. subtilis involves an ordered association of essential primosomal proteins, PriA and DnaD, with the DNA, followed by recruitment of helicase loader proteins, DnaB and DnaI.

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  • Dr. W.K. (Wiep Klaas) Smits | LUMC

    The transcriptional regulator Rok binds A+T-rich DNA and is involved in repression of a mobile genetic element in Bacillus subtilis. PLoS Genet. 2010; 6:e1001207. Smits WK, Goranov AI, Grossman AD. Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo. Mol Microbiol. 2010; 75:452-61.

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  • Structural Analysis of Bacillus subtilis SPP1 Phage

    Apr 25, 2003 · The Bacillus subtilis SPP1 phage-encoded protein G39P is a loader and inhibitor of the phage G40P replicative helicase involved in the initiation of DNA replication.We have carried out a full x-ray crystallographic and preliminary NMR analysis of G39P and functional studies of the protein, including assays for helicase binding by a number of truncated mutant forms, in an effort to improve …

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  • Dr. W.K. (Wiep Klaas) Smits | LUMC

    The transcriptional regulator Rok binds A+T-rich DNA and is involved in repression of a mobile genetic element in Bacillus subtilis. PLoS Genet. 2010; 6:e1001207. Smits WK, Goranov AI, Grossman AD. Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo. Mol Microbiol. 2010; 75:452-61.

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  • How the bacterial replicative helicase opens to start DNA

    Feb 26, 2019 · In a paper published in today's issue of the journal eLife, researchers explain how the helicase loader protein (P loader) from a bacterial virus attaches to …

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  • Viral hijacking of a replicative helicase loader and its

    May 31, 2016 · 6) W. K. Smits, A. I. Goranov, A. D. Grossman, Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo. Mol Microbiol. 75, 452–461 (2010). 7) M. Velten et al., A two-protein strategy for the functional loading of a cellular replicative DNA helicase. Mol Cell. 11, 1009–1020 (2003).

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  • Ordered association of helicase loader proteins with the

    Jan 13, 2010 · Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo Wiep Klaas Smits Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

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  • DnaB, DnaD and DnaI proteins are components of the

    Restart in B. subtilis involves an ordered association of essential primosomal proteins, PriA and DnaD, with the DNA, followed by recruitment of helicase loader proteins, DnaB and DnaI.

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  • Replicative helicase loaders: ring breakers and ring

    Aug 05, 2003 · In Escherichia coli, the replicative DnaB helicase is a stabile hexamer over a wide range of protein concentrations and the E. coli DnaC loader is of the ring breaker class .EcDnaB is capable of self-loading onto single-strand DNA, but does so inefficiently. Efficient loading requires the EcDnaC helicase loader to facilitate opening of the ring, by holding the ring open and/or by increasing

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  • Viral hijacking of a replicative helicase loader and its

    May 31, 2016 · 6) W. K. Smits, A. I. Goranov, A. D. Grossman, Ordered association of helicase loader proteins with the Bacillus subtilis origin of replication in vivo. Mol Microbiol. 75, 452–461 (2010). 7) M. Velten et al., A two-protein strategy for the functional loading of a cellular replicative DNA helicase. Mol Cell. 11, 1009–1020 (2003).

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  • DnaB, DnaD and DnaI proteins are components of the

    Restart in B. subtilis involves an ordered association of essential primosomal proteins, PriA and DnaD, with the DNA, followed by recruitment of helicase loader proteins, DnaB and DnaI.

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  • Regulation of DNA Binding and High-Order Oligomerization

    DnaB is an essential primosomal protein that coloads the replicative helicase in many Gram-positive bacteria, including several human pathogens. Although DnaB is tetrameric in solution, it is from a protein family whose members can oligomerize into large complexes when exposed to DNA. It is currentl …

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