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  • Jaclyn Hayner | PubFacts

    The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA. Authors: The clamp loader uses the energy of ATP binding and hydrolysis to drive conformational changes allowing for β binding and opening, DNA binding, and then release of the β·DNA complex. Although much work has been done studying the sliding

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  • Genes | Special Issue : DNA Replication

    Mar 31, 2015 · Mutation of the glutamate residue in Walker B motifs (or DExx-boxes) in AAA+ proteins typically reduces ATP hydrolysis by as much as a couple orders of magnitude, but has no effect on ATP binding. Here, the Walker B Glu in each of the four active ATP sites of the eukaryotic clamp loader, RFC, was mutated to Gln and Ala separately, and ATP

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  • Alternative clamp loaders/unloaders | FEMS Yeast Research

    Future work is expected to shed more light into the interactions between these fascinating machines. The authors would like to thank past and present members of the Kupiec lab for their work, support and insights. FUNDING. Work on clamp loaders in the lab was supported by the, the and the Volkswagen Foundation. Conflict of interest. None declared.

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  • DNA Replication - PW | Dna Replication | Directionality

    Switch to medium containing only 14N (a light isotope of Nitrogen) Collect sample of cells after various times Analyze the density of the DNA by centrifugation using a CsCl gradient Okazaki Fragments ATP 1 Polymerase III Lagging strand. Helicase + Initiator Proteins. How do the clamp and the clamp loader work together to ensure both

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  • Kinetic analysis of PCNA clamp binding and release in the

    Jan 01, 2015 · The ATP sites of AAA + clamp loaders work together as a switch to assemble clamps on DNA J. Biol. Chem., 289 ( 2014 ), pp. 5537 - 5548 Article Download PDF View Record in …

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  • The ATP sites of AAA+ clamp loaders work together as a

    Feb 28, 2014 · Clamp loaders belong to a family of proteins known as ATPases associated with various cellular activities (AAA+). These proteins utilize the energy from ATP binding and hydrolysis to perform cellular functions. The clamp loader is required to load the clamp onto DNA for use by DNA polymerases to increase processivity.

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  • The ATP Sites of AAA+ Clamp Loaders Work Together as …

    Feb 28, 2014 · Clamp loaders belong to a family of proteins known as ATPases associated with various cellular activities (AAA+). These proteins utilize the energy fr…

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  • Equilibrium ATP titration. A schematic representation of

    The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA.

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  • Kinetic analysis of PCNA clamp binding and release in the

    Jan 01, 2015 · The ATP sites of AAA + clamp loaders work together as a switch to assemble clamps on DNA J. Biol. Chem., 289 ( 2014 ), pp. 5537 - 5548 Article Download PDF View Record in …

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  • (PDF) The ATP sites of AAA+ clamp loaders work …

    The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA. The Journal of biological chemistry. 289(9):5537-48 [DOI] 10.1074/jbc.M113.541466. [PMID] 24436332.

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  • Frontiers | Replisome Assembly at Bacterial Chromosomes

    The ATPase activity of the clamp loader is lower when it is assembled at the 5′ terminus, comparing to the ATPase activity of clamp loader located at 3′ terminus (Park and O'Donnell, 2009). ATP hydrolysis triggers β-clamp closing on DNA and the release of clamp loader from β-clamp:DNA nucleoprotein complex (Pietroni and von Hippel, 2008).

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  • Alternative clamp loaders/unloaders | FEMS Yeast Research

    Future work is expected to shed more light into the interactions between these fascinating machines. The authors would like to thank past and present members of the Kupiec lab for their work, support and insights. FUNDING. Work on clamp loaders in the lab was supported by the, the and the Volkswagen Foundation. Conflict of interest. None declared.

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  • Bacterial and Eukaryotic Replisome Machines

    Sliding clamps do not assemble onto DNA by themselves; they require a multi‐subunit clamp loader that uses ATP to open . and close the ring around DNA [7]. Clamp loaders of all cells are composed of 5 homologous subunits, and each subunit is a member of the …

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  • Linda B Bloom » Department of Biochemistry and Molecular

    Error bars represent S.D. from publication: The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA | Clamp loaders belong to a family of proteins known as AAA+

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  • Jaclyn Hayner - Publications

    The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA. The Journal of Biological Chemistry. 289: 5537-48. PMID 24436332 DOI: 10.1074/jbc.M113.541466 : 0.96: 2013: Hayner JN, Bloom LB. The β sliding clamp closes around DNA prior to release by the Escherichia coli clamp loader γ complex.

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  • The ATP Sites of AAA+ Clamp Loaders Work Together as a

    Feb 28, 2014 · The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA. Marzahn MR (1), Hayner JN, Finkelstein J, O'Donnell M, Bloom LB. Clamp loaders belong to a family of proteins known as ATPases associated with various cellular activities (AAA+).

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  • The ATP sites of AAA+ clamp loaders work together as a

    The clamp loader is required to load the clamp onto DNA for use by DNA polymerases to inc … The ATP sites of AAA+ clamp loaders work together as a switch to assemble clamps on DNA J Biol Chem .

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  • The regulatory function of the AAA4 ATPase domain of

    Nov 23, 2020 · Cytoplasmic dynein is the primary motor for microtubule minus-end-directed transport and is indispensable to eukaryotic cells. Although each motor domain of dynein contains three active AAA+

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  • Machinery of DNA Replication - Rockefeller University

    Sliding clamps are ring-shaped proteins that encircle primed sites and directly bind the DNA polymerases, holding them to DNA in a mobile fashion for the high processivity required to replicate long genomes. The clamps are placed around DNA by a clamp loader that couples ATP to …

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  • The Clamp loader of Escherichia coli DNA Polymerase III

    The x and vp Subunits are E. coli Clamp Loader AAA+ Adaptor Proteins 237 of how complex multi-protein machines interact and work together in a well-regulated the clamp loader is a molecular switch, activated by ATP binding, and inactivated by DNA-dependent hydrolysis of ATP. The 20

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